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Journal: BioMed Research International
Article Title: Excess Hydrocortisone Hampers Placental Nutrient Uptake Disrupting Cellular Metabolism
doi: 10.1155/2018/5106174
Figure Lengend Snippet: Assessment of hydrocortisone-induced cellular death by apoptosis in healthy-term placental explants. (a) Representative microphotographs of TUNEL assay to determine apoptosis in each experimental condition; cells undergoing apoptosis present fluorescent green nuclei. (b) Quantification of the % of TUNEL positive cells per condition. (c) Active caspases 3 and 7 as measured by luminometry, using the Caspase Glo Assay. (d) Cleaved caspase 3 as determined by western blot and normalized by actin levels. Values are presented as means ± SEM for 5 independent experiments. ∗ p≤0.05; ∗∗ p≤0.01; ∗∗∗ p≤0.001 relative to control.
Article Snippet: Apoptosis was assessed with a commercially available
Techniques: TUNEL Assay, Caspase-Glo Assay, Western Blot, Control